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Plant Physiol Biochem. 2014 Feb;75:128-37. doi: 10.1016/j.plaphy.2013.12.008. Epub 2014 Jan 4.

TR-DB: an open-access database of compounds affecting the ethylene-induced triple response in Arabidopsis.

Author information

1
Laboratory of Functional Plant Biology, Department of Physiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: yuming.hu@ugent.be.
2
Laboratory of Functional Plant Biology, Department of Physiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: pieter.callebert@ugent.be.
3
Direction Information and Communication Technology, Ghent University, Krijgslaan 281, B-9000 Gent, Belgium. Electronic address: ilse.vandemoortel@ugent.be.
4
VIB, Compound Screening Facility, Technologiepark 927, B-9052 Zwijnaarde, Belgium. Electronic address: longu@psb.vib-ugent.be.
5
VIB, Compound Screening Facility, Technologiepark 927, B-9052 Zwijnaarde, Belgium. Electronic address: doaud@psb.vib-ugent.be.
6
Direction Information and Communication Technology, Ghent University, Krijgslaan 281, B-9000 Gent, Belgium. Electronic address: luc.verschraegen@ugent.be.
7
Laboratory of Functional Plant Biology, Department of Physiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: filip.vandenbussche@ugent.be.
8
Laboratory of Functional Plant Biology, Department of Physiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: dominique.vanderstraeten@ugent.be.

Abstract

Small molecules which act as hormone agonists or antagonists represent useful tools in fundamental research and are widely applied in agriculture to control hormone effects. High-throughput screening of large chemical compound libraries has yielded new findings in plant biology, with possible future applications in agriculture and horticulture. To further understand ethylene biosynthesis/signaling and its crosstalk with other hormones, we screened a 12,000 compound chemical library based on an ethylene-related bioassay of dark-grown Arabidopsis thaliana (L.) Heynh. seedlings. From the initial screening, 1313 (∼11%) biologically active small molecules altering the phenotype triggered by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), were identified. Selection and sorting in classes were based on the angle of curvature of the apical hook, the length and width of the hypocotyl and the root. A MySQL-database was constructed (https://chaos.ugent.be/WE15/) including basic chemical information on the compounds, images illustrating the phenotypes, phenotype descriptions and classification. The research perspectives for different classes of hit compounds will be evaluated, and some general screening tips for customized high-throughput screening and pitfalls will be discussed.

KEYWORDS:

1-aminocyclopropane-1-carboxylic acid (ACC); Arabidopsis; Chemical genetics; Database; Ethylene; High throughput phenotypic screen; Triple response

PMID:
24441765
DOI:
10.1016/j.plaphy.2013.12.008
[Indexed for MEDLINE]

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