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Items: 1 to 20 of 44

1.

Dynamics, ultrastructure and gene expression of human in vitro organized testis cells from testicular sperm extraction biopsies.

von Kopylow K, Schulze W, Salzbrunn A, Schaks M, Schäfer E, Roth B, Schlatt S, Spiess AN.

Mol Hum Reprod. 2018 Mar 1;24(3):123-134. doi: 10.1093/molehr/gax070.

PMID:
29304256
2.

Human spermatogonial markers.

von Kopylow K, Spiess AN.

Stem Cell Res. 2017 Dec;25:300-309. doi: 10.1016/j.scr.2017.11.011. Epub 2017 Nov 15. Review.

3.

Enabling reproducible real-time quantitative PCR research: the RDML package.

Rödiger S, Burdukiewicz M, Spiess AN, Blagodatskikh K.

Bioinformatics. 2017 Dec 15;33(24):4012-4014. doi: 10.1093/bioinformatics/btx528.

PMID:
28961912
4.

System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation.

Spiess AN, Rödiger S, Burdukiewicz M, Volksdorf T, Tellinghuisen J.

Sci Rep. 2016 Dec 13;6:38951. doi: 10.1038/srep38951.

5.

Comment on "Absence of sperm RNA elements correlates with idiopathic male infertility".

Cappallo-Obermann H, Spiess AN.

Sci Transl Med. 2016 Aug 24;8(353):353tc1. doi: 10.1126/scitranslmed.aaf2396.

PMID:
27559097
6.

The impact of sperm protamine deficiency and sperm DNA damage on human male fertility: a systematic review and meta-analysis.

Ni K, Spiess AN, Schuppe HC, Steger K.

Andrology. 2016 Sep;4(5):789-99. doi: 10.1111/andr.12216. Epub 2016 May 27. Review.

7.

Isolation and gene expression analysis of single potential human spermatogonial stem cells.

von Kopylow K, Schulze W, Salzbrunn A, Spiess AN.

Mol Hum Reprod. 2016 Apr;22(4):229-39. doi: 10.1093/molehr/gaw006. Epub 2016 Jan 20.

PMID:
26792870
8.

GnT1IP-L specifically inhibits MGAT1 in the Golgi via its luminal domain.

Huang HH, Hassinen A, Sundaram S, Spiess AN, Kellokumpu S, Stanley P.

Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08916.

9.

Bias and imprecision in analysis of real-time quantitative polymerase chain reaction data.

Tellinghuisen J, Spiess AN.

Anal Chem. 2015 Sep 1;87(17):8925-31. doi: 10.1021/acs.analchem.5b02057. Epub 2015 Aug 13.

PMID:
26235706
10.

Absolute copy number from the statistics of the quantification cycle in replicate quantitative polymerase chain reaction experiments.

Tellinghuisen J, Spiess AN.

Anal Chem. 2015 Feb 3;87(3):1889-95. doi: 10.1021/acs.analchem.5b00077. Epub 2015 Jan 23.

PMID:
25582662
11.

Impact of smoothing on parameter estimation in quantitative DNA amplification experiments.

Spiess AN, Deutschmann C, Burdukiewicz M, Himmelreich R, Klat K, Schierack P, Rödiger S.

Clin Chem. 2015 Feb;61(2):379-88. doi: 10.1373/clinchem.2014.230656. Epub 2014 Dec 4.

12.

Statistical uncertainty and its propagation in the analysis of quantitative polymerase chain reaction data: comparison of methods.

Tellinghuisen J, Spiess AN.

Anal Biochem. 2014 Nov 1;464:94-102. doi: 10.1016/j.ab.2014.06.015. Epub 2014 Jun 30.

PMID:
24991688
13.

Comparing real-time quantitative polymerase chain reaction analysis methods for precision, linearity, and accuracy of estimating amplification efficiency.

Tellinghuisen J, Spiess AN.

Anal Biochem. 2014 Mar 15;449:76-82. doi: 10.1016/j.ab.2013.12.020. Epub 2013 Dec 21.

PMID:
24365068
14.

The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme.

Rogenhofer N, Dansranjavin T, Schorsch M, Spiess A, Wang H, von Schönfeldt V, Cappallo-Obermann H, Baukloh V, Yang H, Paradowska A, Chen B, Thaler CJ, Weidner W, Schuppe HC, Steger K.

Hum Reprod. 2013 Apr;28(4):969-78. doi: 10.1093/humrep/des471. Epub 2013 Jan 22.

PMID:
23340056
15.

Fold-change correction values for testicular somatic transcripts in gene expression studies of human spermatogenesis.

Cappallo-Obermann H, Feig C, Schulze W, Spiess AN.

Hum Reprod. 2013 Mar;28(3):590-8. doi: 10.1093/humrep/des433. Epub 2013 Jan 9.

PMID:
23303554
16.

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications.

Ruijter JM, Pfaffl MW, Zhao S, Spiess AN, Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De Preter K, Derveaux S, Hellemans J, Vandesompele J.

Methods. 2013 Jan;59(1):32-46. doi: 10.1016/j.ymeth.2012.08.011. Epub 2012 Sep 3.

PMID:
22975077
17.

Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development.

Paradowska AS, Miller D, Spiess AN, Vieweg M, Cerna M, Dvorakova-Hortova K, Bartkuhn M, Schuppe HC, Weidner W, Steger K.

Epigenetics. 2012 Sep;7(9):1057-70. Epub 2012 Aug 16.

18.

Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia.

von Kopylow K, Staege H, Spiess AN, Schulze W, Will H, Primig M, Kirchhoff C.

Reproduction. 2012 Jan 1;143(1):45-57. doi: 10.1530/REP-11-0290. Epub 2011 Nov 1.

PMID:
22046055
19.

A one-step real-time multiplex PCR for screening Y-chromosomal microdeletions without downstream amplicon size analysis.

Kozina V, Cappallo-Obermann H, Gromoll J, Spiess AN.

PLoS One. 2011;6(8):e23174. doi: 10.1371/journal.pone.0023174. Epub 2011 Aug 22.

20.

Highly purified spermatozoal RNA obtained by a novel method indicates an unusual 28S/18S rRNA ratio and suggests impaired ribosome assembly.

Cappallo-Obermann H, Schulze W, Jastrow H, Baukloh V, Spiess AN.

Mol Hum Reprod. 2011 Nov;17(11):669-78. doi: 10.1093/molehr/gar037. Epub 2011 May 18.

PMID:
21593214

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