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Neurosci Res. 2014 Apr-May;81-82:11-20. doi: 10.1016/j.neures.2014.03.006. Epub 2014 Mar 31.

Serotonin 2A receptor regulates microtubule assembly and induces dynamics of dendritic growth cones in rat cortical neurons in vitro.

Author information

1
University of Tsukuba, Graduate School of Comprehensive Human Sciences, 1-1-1 Tennodai, Tsukuba 305-8577, Japan.
2
University of Tsukuba, Graduate School of Comprehensive Human Sciences, 1-1-1 Tennodai, Tsukuba 305-8577, Japan. Electronic address: tshiga@md.tsukuba.ac.jp.

Abstract

Serotonin (5-HT) regulates the development of cerebral cortex, but 5-HT receptors mediating the effects are poorly understood. We investigated roles of 5-HT2A receptor in dendritic growth cones using dissociation culture of rat cerebral cortex. Neurons at embryonic day 16 were cultured for 4 days and treated with 5-HT2A/2C receptor agonist (DOI) for 4h. DOI increased the size of growth cone periphery which was actin-rich and microtubule-associated protein 2-negative at the dendritic tip. The length increase of the growth cone periphery may be mediated by 5-HT2A receptor, because the 5-HT2A receptor antagonist reversed the effects of DOI. Moreover, the time-lapse analysis demonstrated the increase of morphological dynamics in dendritic growth cones by DOI. Next, to elucidate the mechanisms underlying the actions of 5-HT2A receptor in dendritic growth cones, we examined the cytoskeletal proteins, tyrosinated α-tubulin (Tyr-T; dynamic tubulin) and acetylated α-tubulin (Ace-T; stable tubulin). DOI increased the fluorescence intensity of Tyr-T, while decreased that of Ace-T in the dendritic growth cone periphery. These effects were reversed by the 5-HT2A receptor antagonist, suggesting that 5-HT2A receptor promotes microtubule dynamics. In summary, it was suggested that 5-HT2A receptor induces morphological changes and dynamics of dendritic growth cones through regulation of microtubule assembly.

KEYWORDS:

Cerebral cortex; Cytoskeleton; Dendrite; Serotonin

PMID:
24698813
DOI:
10.1016/j.neures.2014.03.006
[Indexed for MEDLINE]

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