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Angew Chem Int Ed Engl. 2019 Feb 1. doi: 10.1002/anie.201814266. [Epub ahead of print]

Selective Engineering of Linkage-Specific α2,6-N-Linked Sialoproteins Using Sydnone-Modified Sialic Acid Bioorthogonal Reporters.

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Institut Européen de Chimie et Biologie, Université de Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.
Institut de Neurosciences Cognitives et Intégratives d'Aquitaine, CNRS UMR5287, Bordeaux, France.
Institut Bergonié, INSERM U1218, Bordeaux, France.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Current address: Centro Andaluz de Biología Molecular y Medicina Regenerativa, Consejo Superior de Investigaciones Científicas-, Universidad de Sevilla-Universidad Pablo de Olavide, Avda. Américo Vespucio 24, 41092, Seville, Spain.


The metabolic oligosaccharide engineering (MOE) strategy using unnatural sialic acids has recently enabled the visualization of the sialome in living systems. However, MOE only reports on global sialylation and dissected information regarding subsets of sialosides is missing. Described here is the synthesis and utilization of sialic acids modified with a sydnone reporter for the metabolic labeling of sialoconjugates. The positioning of the reporter on the sugar significantly altered its metabolic fate. Further in vitro enzymatic assays revealed that the 9-modified neuraminic acid is preferentially accepted by the sialyltransferase ST6Gal-I over ST3Gal-IV, leading to the favored incorporation of the reporter into linkage-specific α2,6-N-linked sialoproteins. This sydnone sugar presents the possibility of investigating the roles of specific sialosides.


click chemistry; enzymes; fluorescent probes; glycobiology; sialic acids


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