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Nat Commun. 2018 Jul 27;9(1):2939. doi: 10.1038/s41467-018-05278-2.

Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum.

Author information

1
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
2
Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Dr. Aiguader, 88, 08003, Barcelona, Spain.
3
Universitat Pompeu Fabra (UPF), Dr. Aiguader, 88, 08003, Barcelona, Spain.
4
Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark.
5
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
6
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. pedro.carvalho@path.ox.ac.uk.
7
Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Dr. Aiguader, 88, 08003, Barcelona, Spain. pedro.carvalho@path.ox.ac.uk.
8
Universitat Pompeu Fabra (UPF), Dr. Aiguader, 88, 08003, Barcelona, Spain. pedro.carvalho@path.ox.ac.uk.

Abstract

Lipid droplets (LDs) and peroxisomes are ubiquitous organelles with central roles in eukaryotic cells. Although the mechanisms involved in biogenesis of these organelles remain elusive, both seem to require the endoplasmic reticulum (ER). Here we show that in yeast the ER budding of these structurally unrelated organelles has remarkably similar requirements and involves cooperation between Pex30 and the seipin complex. In the absence of these components, budding of both LDs and peroxisomes is inhibited, leading to the ER accumulation of their respective constituent molecules, such as triacylglycerols and peroxisomal membrane proteins, whereas COPII vesicle formation remains unaffected. This phenotype can be reversed by remodeling ER phospholipid composition highlighting a key function of these lipids in organelle biogenesis. We propose that seipin and Pex30 act in concert to organize membrane domains permissive for organelle budding, and that may have a lipid composition distinct from the bulk ER.

PMID:
30054465
PMCID:
PMC6063905
DOI:
10.1038/s41467-018-05278-2
[Indexed for MEDLINE]
Free PMC Article

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