Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Methods Mol Biol. 2017;1610:123-133. doi: 10.1007/978-1-4939-7003-2_9.

Sample Preparation Protocols for Protein Abundance, Acetylome, and Phosphoproteome Profiling of Plant Tissues.

Author information

1
Department of Plant Pathology and Microbiology, Iowa State University, 43 Bessey, Ames, IA, 50011, USA.
2
Department of Plant Pathology and Microbiology, Iowa State University, 43 Bessey, Ames, IA, 50011, USA. jwalley@iastate.edu.

Abstract

Peptide mass spectrometry is an invaluable technique to globally quantify the proteome. Central to proteome profiling are efficient methods to extract proteins, digest proteins into peptides, and enrich for posttranslationally modified peptides prior to mass spectrometry. In this chapter, we describe methods to extract proteins, process them into peptides, and optionally enrich for phospho- and acetyl-peptides prior to analysis by mass spectrometry.

KEYWORDS:

Acetylome; Mass spectrometry; Phosphoproteome; Plant proteomics; Protein extraction

PMID:
28439861
DOI:
10.1007/978-1-4939-7003-2_9
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center