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Nat Med. 2018 May;24(4):438-449. doi: 10.1038/nm.4491. Epub 2018 Mar 12.

Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization.

Guo N1,2,3, Soden ME4,5, Herber C1,2, Kim MT1,2,3, Besnard A1,2,3, Lin P1,2,3, Ma X6,7, Cepko CL6,7, Zweifel LS4,5, Sahay A1,2,3,8.

Author information

Center for Regenerative Medicine, Massachusetts General Hospital (MGH), Boston, Massachusetts, USA.
Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
Department of Psychiatry, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Department of Pharmacology, University of Washington, Seattle, Washington, USA.
Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington, USA.
Howard Hughes Medical Institute, Boston, Massachusetts, USA.
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
BROAD Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.


Memories become less precise and generalized over time as memory traces reorganize in hippocampal-cortical networks. Increased time-dependent loss of memory precision is characterized by an overgeneralization of fear in individuals with post-traumatic stress disorder (PTSD) or age-related cognitive impairments. In the hippocampal dentate gyrus (DG), memories are thought to be encoded by so-called 'engram-bearing' dentate granule cells (eDGCs). Here we show, using rodents, that contextual fear conditioning increases connectivity between eDGCs and inhibitory interneurons (INs) in the downstream hippocampal CA3 region. We identify actin-binding LIM protein 3 (ABLIM3) as a mossy-fiber-terminal-localized cytoskeletal factor whose levels decrease after learning. Downregulation of ABLIM3 expression in DGCs was sufficient to increase connectivity with CA3 stratum lucidum INs (SLINs), promote parvalbumin (PV)-expressing SLIN activation, enhance feedforward inhibition onto CA3 and maintain a fear memory engram in the DG over time. Furthermore, downregulation of ABLIM3 expression in DGCs conferred conditioned context-specific reactivation of memory traces in hippocampal-cortical and amygdalar networks and decreased fear memory generalization at remote (i.e., distal) time points. Consistent with the observation of age-related hyperactivity of CA3, learning failed to increase DGC-SLIN connectivity in 17-month-old mice, whereas downregulation of ABLIM3 expression was sufficient to restore DGC-SLIN connectivity, increase PV+ SLIN activation and improve the precision of remote memories. These studies exemplify a connectivity-based strategy that targets a molecular brake of feedforward inhibition in DG-CA3 and may be harnessed to decrease time-dependent memory generalization in individuals with PTSD and improve memory precision in aging individuals.

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