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14,263 results

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Page 1
Analysis of Schizosaccharomyces pombe Meiosis.
Yamashita A, Sakuno T, Watanabe Y, Yamamoto M. Yamashita A, et al. Cold Spring Harb Protoc. 2017 Sep 1;2017(9):pdb.top079855. doi: 10.1101/pdb.top079855. Cold Spring Harb Protoc. 2017. PMID: 28733417 Review.
S. pombe cells, which normally grow in the haploid state, diploidize by conjugation and initiate meiosis when starved for nutrients, especially nitrogen. ...Mei2 is also a key factor for meiosis-specific gene expression. Studies on S. pombe have offere …
S. pombe cells, which normally grow in the haploid state, diploidize by conjugation and initiate meiosis when starved for nutr …
Schizosaccharomyces pombe Isolation Protocol.
Benito Á, Calderón F, Benito S. Benito Á, et al. Methods Mol Biol. 2018;1721:227-234. doi: 10.1007/978-1-4939-7546-4_20. Methods Mol Biol. 2018. PMID: 29423861
This chapter describes a methodology to isolate yeast strains from Schizosaccharomyces pombe species. The method is based on a selective-differential medium that notably facilitates the isolation of S. pombe. ...Several selective-differential agents based on …
This chapter describes a methodology to isolate yeast strains from Schizosaccharomyces pombe species. The method is based on a select …
Application of the fission yeast Schizosaccharomyces pombe in human nutrition.
Chen ES. Chen ES. FEMS Yeast Res. 2023 Jan 4;23:foac064. doi: 10.1093/femsyr/foac064. FEMS Yeast Res. 2023. PMID: 36574952 Review.
Fission yeast Schizosaccharomyces pombe (S. pombe) is renowned as a powerful genetic model for deciphering cellular and molecular biological phenomena, including cell division, chromosomal events, stress responses, and human carcinogenesis. Traditionally, Afr …
Fission yeast Schizosaccharomyces pombe (S. pombe) is renowned as a powerful genetic model for deciphering cellular and …
High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe.
Rai SK, Atwood-Moore A, Levin HL. Rai SK, et al. Methods Mol Biol. 2018;1721:167-177. doi: 10.1007/978-1-4939-7546-4_15. Methods Mol Biol. 2018. PMID: 29423856 Free PMC article. Review.
We describe here an efficient transformation procedure for use in the fission yeast Schizosaccharomyces pombe. This method relies on chemical agents (lithium acetate, and polyethylene glycol) and temperature stresses, which ultimately facilitate transfer of the genetic mat …
We describe here an efficient transformation procedure for use in the fission yeast Schizosaccharomyces pombe. This method relies on …
Extension of chronological lifespan in Schizosaccharomyces pombe.
Ohtsuka H, Shimasaki T, Aiba H. Ohtsuka H, et al. Genes Cells. 2021 Jul;26(7):459-473. doi: 10.1111/gtc.12854. Epub 2021 May 12. Genes Cells. 2021. PMID: 33977597 Free PMC article. Review.
The present microreview focuses on aging and longevity studies, particularly on chronological lifespan (CLS) concerning the unicellular eukaryotic fission yeast Schizosaccharomyces pombe. In S. pombe, >30 compounds, 8 types of nutrient restriction, and &gt …
The present microreview focuses on aging and longevity studies, particularly on chronological lifespan (CLS) concerning the unicellular euka …
Metabolism of phospholipids in the yeast Schizosaccharomyces pombe.
Holič R, Pokorná L, Griač P. Holič R, et al. Yeast. 2020 Jan;37(1):73-92. doi: 10.1002/yea.3451. Epub 2019 Dec 22. Yeast. 2020. PMID: 31785604 Review.
In this review, we describe the biosynthetic pathways leading to major S. pombe phospholipids, how these pathways are regulated, and what is known about degradation and turnover of fission yeast phospholipids. This review also addresses the synthesis, regulation and …
In this review, we describe the biosynthetic pathways leading to major S. pombe phospholipids, how these pathways are regulate …
Genes affecting the extension of chronological lifespan in Schizosaccharomyces pombe (fission yeast).
Ohtsuka H, Shimasaki T, Aiba H. Ohtsuka H, et al. Mol Microbiol. 2021 Apr;115(4):623-642. doi: 10.1111/mmi.14627. Epub 2020 Nov 3. Mol Microbiol. 2021. PMID: 33064911 Free PMC article. Review.
We describe the signal transduction pathways that affect CLS in S. pombe: target of rapamycin complex 1, cAMP-dependent protein kinase, Sty1, and Pmk1 pathways have important functions in the regulation of CLS extension. ...In this review, we focus on the individual …
We describe the signal transduction pathways that affect CLS in S. pombe: target of rapamycin complex 1, cAMP-dependent protei …
The phenomenon of lipid metabolism "cut" mutants.
Zach R, Převorovský M. Zach R, et al. Yeast. 2018 Dec;35(12):631-637. doi: 10.1002/yea.3358. Epub 2018 Oct 29. Yeast. 2018. PMID: 30278108 Review.
Recently, several studies established a functional link between biosynthesis of structural phospholipids, nuclear membrane growth, and the fidelity of "closed" mitosis in S. pombe. These novel insights suggest a mechanistic explanation for the mitotic defects charac …
Recently, several studies established a functional link between biosynthesis of structural phospholipids, nuclear membrane growth, and the f …
Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A, Elgort MG, Brandman O, Ives C, Collins SR, Miller-Vedam L, Weibezahn J, Hein MY, Poser I, Mann M, Hyman AA, Weissman JS. Frost A, et al. Cell. 2012 Jun 8;149(6):1339-52. doi: 10.1016/j.cell.2012.04.028. Cell. 2012. PMID: 22682253 Free PMC article.
We present a genetic interaction map of pairwise measures including 40% of nonessential S. pombe genes. By comparing interaction maps for fission and budding yeast, we confirmed widespread conservation of genetic relationships within and between complexes and pathwa …
We present a genetic interaction map of pairwise measures including 40% of nonessential S. pombe genes. By comparing interacti …
Genetic Analysis of Schizosaccharomyces pombe.
Ekwall K, Thon G. Ekwall K, et al. Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.top079772. doi: 10.1101/pdb.top079772. Cold Spring Harb Protoc. 2017. PMID: 28765303
In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic comple …
In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pomb …
14,263 results
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