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Reprod Sci. 2014 Nov;21(11):1362-9. doi: 10.1177/1933719114525271. Epub 2014 Mar 6.

Resveratrol and endometrium: a closer look at an active ingredient of red wine using in vivo and in vitro models.

Author information

1
Department of Obstetrics and Gynecology, Greenville Health System, Greenville, SC, USA.
2
Departmento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Brazil Department of Obstetrics and Gynecology, University of North Carolina at Chapel Hill, NC, USA.
3
Department of Obstetrics and Gynecology, Greenville Health System, Greenville, SC, USA Department of Biology, Furman University, Greenville, SC, USA.
4
Department of Obstetrics and Gynecology, University of North Carolina at Chapel Hill, NC, USA.
5
Department of Obstetrics and Gynecology, Greenville Health System, Greenville, SC, USA blessey@ghs.org.

Abstract

Resveratrol is a natural phytoestrogen with antiproliferative properties present in red wine, grapes, and berries. Published reports on the effects of resveratrol in human endometrial function are limited. The objective of this study was to investigate the expression of estrogen receptor α (ESR1), Ki-67 (a proliferative marker), aryl hydrocarbon receptor (AhR), and members of the cytochrome P450 superfamily of enzymes (CYP1A1 and CYP1B1) in an in vitro and vivo assay. Alkaline phosphatase assay of estrogenicity was used to compare estrogen activity of different concentrations of resveratrol to estradiol (E2) and diethylstilbestrol (DES), using Ishikawa cell culture. Immunohistochemical expression of ESR1 and Ki67, and reverse transcriptase polymerase chain reaction of AhR, CYP1A1, and CYP1B1 were analyzed from xenograft implants of human endometrial tissue in ovariectomized immunodeficient RAG-2-γ(c) mice, after 30 days of treatment with subcutaneous pellets of E2, E2 plus progesterone (P4), or E2 plus resveratrol (6, 30, or 60 mg) for 30 days. Compared to E2, resveratrol acted as an agonist and antagonist of estrogen in low and high concentrations, respectively, when combined with E2. Xenografts of human endometrial tissues in RAG-2 mice exhibited reduced expression of ESR1 and proliferative activity (Ki67) with 60 mg of resveratrol. This study suggests that resveratrol, at high doses, has the potential benefit to reduce proliferation of human endometrium through ESR1.

KEYWORDS:

Ishikawa; cell proliferation; endometriosis; estrogen receptor; human endometrium; resveratrol

PMID:
24604232
PMCID:
PMC4212329
DOI:
10.1177/1933719114525271
[Indexed for MEDLINE]
Free PMC Article

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