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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Dec;31(12):1615-9.

[Recombinant influenza A virus NS2 protein inhibits the production of interferon in lung tissues of infected mice].

[Article in Chinese]

Author information

1
Department of Microbiology, Guizhou Medical University, Guiyang 550004, China.
2
Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China.
3
[1]Department of Microbiology, [2]Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China. *Corresponding author, E-mail: 624057523@qq.com.

Abstract

OBJECTIVE:

To obtain non-structural protein 2 (NS2) of influenza A virus (IAV) via the expression system of Escherichia coli, and observe the effect of NS2 on the production of interferon γ (IFN-γ) in mouse lung tissues.

METHODS:

A prokaryotic expression plasmid pET22b(+)/NS2 was constructed and transformed into Rosetta-gami(2) of Escherichia coli. Through the inducible expression and purification, NS2 was prepared. Forty BALB/c mice were divided randomly into 8 groups, normal control group, 200 μg/kg NS2 joint virus group, 200 μg/kg NS2 joint RNA group, 100 μg/kg inactivated virus group, 0.2×lethal dose 50% (LD50) virus group, viral RNA group of 50 μg/kg, 200 μg/kg NS2 group, and 100 μg/kg NS2 group. All mice were subjected to nasal inhalation. Reverse transcription-PCR and Western blotting were respectively performed to detect the mRNA and protein expressions of IFN-γ in lung tissues.

RESULTS:

Recombinant NS2 was obtained by the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) and purification. After the nasal inhalation of NS2 or/and IAV to mice, reverse transcription-PCR and Western blotting showed that the levels of IFN-γ mRNA and protein in the lung tissues of NS2 joint virus group were lower than those of the virus group.

CONCLUSION:

NS2 could inhibit the expressions of IFN-γ mRNA and protein in lung tissues of mice infected by IAV.

PMID:
26648293
[Indexed for MEDLINE]

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