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J Virol Methods. 2013 Apr;189(1):232-4. doi: 10.1016/j.jviromet.2013.01.019. Epub 2013 Feb 4.

Rapid deep sequencing of patient-derived HIV with ion semiconductor technology.

Author information

1
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, United States.

Abstract

The development of next-generation sequencing technologies has facilitated the study of HIV drug resistance evolution. However, the high capacity and per-run cost of many sequencers is not ideal for viral sequencing unless many samples are analyzed simultaneously. Ion semiconductor sequencing has recently emerged as a flexible, lower-cost alternative with short runtime. This paper describes the use of Ion Torrent devices for deep sequencing of drug resistant HIV samples. High levels of sequencing coverage were obtained in HIV Gag and protease, allowing the detection of mutations at low frequencies.

PMID:
23384677
PMCID:
PMC3608812
DOI:
10.1016/j.jviromet.2013.01.019
[Indexed for MEDLINE]
Free PMC Article

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