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Transfusion. 2013 Apr;53(4):741-6. doi: 10.1111/j.1537-2995.2012.03800.x. Epub 2012 Jul 13.

RHCE*ceTI encodes partial c and partial e and is often in cis to RHD*DIVa.

Author information

1
Laboratory of Immunohematology and Genomics, New York Blood Center, Long Island City, New York 10065, USA. mreid@nybloodcenter.org

Abstract

BACKGROUND:

In the Rh blood group system, variant RhD and RhCE express several partial antigens. We investigated RH in samples with partial DIVa that demonstrated weak and variable reactivity with anti-C.

STUDY DESIGN AND METHODS:

Standard hemagglutination techniques, polymerase chain reaction-based assays, and RH sequencing were used.

RESULTS:

DNA analysis showed that six red blood cell (RBC) samples with weak and inconsistent reactivity with anti-C lacked RHCE*C, but all had RHD*DIVa, which encodes partial D and Go(a) . We then tested RBCs from 19 Go(a+) cryopreserved samples (confirmed to have RHD*DIVa) with four anti-C and observed weak variable reactions. RHCE genotyping found all but one of the samples with RHD*DIVa also had RHCE nt 48G>C and 1025C>T, named RHCE*ceTI. Lookback of samples referred for workup and found to have either allele revealed 47 of 55 had both RHD*DIVa and RHCE*ceTI, four had RHD*DIVa without RHCE*ceTI, and four had RHCE*ceTI without RHD*DIVa. Alloanti-c was found in a patient with c+ RBCs and RHCE*ceTI in trans to RHCE*Ce, and alloanti-e was found in a patient with e+ RBC and RHCE*ceTI in trans to RHCE*cE. RHD*DIVa in trans to RHD erroneously tested as RHD hemizygous.

CONCLUSIONS:

RHD*DIVa and RHCE*ceTI almost always, but not invariably, travel together. This haplotype is found in people of African ancestry and the RBCs can demonstrate aberrant reactivity with anti-C. RHCE*ceTI encodes partial c and e antigens. We confirm that RHD zygosity assays are unreliable in samples with RHD*DIVa.

PMID:
22804620
PMCID:
PMC3477252
DOI:
10.1111/j.1537-2995.2012.03800.x
[Indexed for MEDLINE]
Free PMC Article

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