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Lett Appl Microbiol. 2012 Jun;54(6):537-42. doi: 10.1111/j.1472-765X.2012.03243.x. Epub 2012 Apr 10.

Quantifying dental biofilm growth using cross-polarization optical coherence tomography.

Author information

1
School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA.

Abstract

AIMS:

Quantifying the ex vivo growth of complex multispecies dental biofilms using cross-polarization 1310-nm optical coherence tomography (CP-OCT) system was investigated.

METHODS AND RESULTS:

Bacterial microcosms, which were derived from plaque samples of paediatric subjects, were incubated in a biofilm reactor system containing discs of different dental materials for 72 h with daily sucrose pulsing (5×). CP-OCT analysis of biofilm mass was validated with crystal violet (CV) assays at various growth stages of these complex biofilms. CP-OCT was able to filter out the back-reflected signals of water layers in the hydrated biofilm and allowed for direct biofilm quantification. The overall depth-resolved scattering intensity of the biofilm showed very strong positive correlation with CV assay quantification (Spearman's ρ = 0.92) during the growth phase of the biofilm.

CONCLUSION:

CP-OCT was able to quantify the mass of the biofilm by measuring the overall depth-resolved scattering of the biofilm.

SIGNIFICANCE AND IMPACT OF THE STUDY:

CP-OCT has the ability to nondestructively monitor biofilm growth and elucidate the growth characteristics of these microcosms on different dental material compositions.

PMID:
22435929
PMCID:
PMC3351576
DOI:
10.1111/j.1472-765X.2012.03243.x
[Indexed for MEDLINE]
Free PMC Article

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