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Sci Rep. 2015 Sep 23;5:13222. doi: 10.1038/srep13222.

QIAD assay for quantitating a compound's efficacy in elimination of toxicoligomers.

Author information

1
Institute of Complex Systems, Structural Biochemistry (ICS-6), Research Centre Jülich, 52425 Jülich, Germany.
2
Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
3
Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
4
Institute of Neuroscience and Medicine (INM-4), Research Centre Jülich (FZJ), 52425 Jülich, Germany.
5
Institute for Bioorganic Chemistry, Heinrich-Heine-Universität Düsseldorf, 52426 Jülich, Germany.
6
Institut für Bio- und Geowissenschaften: Biotechnologie (IBG-1), Forschungszentrum Jülich, 52428 Jülich, Germany.
7
Fraunhofer Institute for Cell Therapy and Immunology, Dep. Molecular Drug Biochemistry and Therapy, 06120 Halle, Germany.
8
Institute of Mathematics, Lehrstuhl für Statistik und Wahrscheinlichkeitstheorie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
9
Bioanalytik, Hochschule für Angewandte Wissenschaften, Coburg, Germany.

Abstract

Strong evidence exists for a central role of amyloid β-protein (Aβ) oligomers in the pathogenesis of Alzheimer's disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the Aβ aggregate size distribution. Applying QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the benzofuran derivative KMS88009, ZAβ3W, the D-enantiomeric peptide D3 and its tandem version D3D3 on Aβ aggregation. The predictive power of the assay for in vivo efficacy is demonstrated by comparing the oligomer elimination efficiency of D3 and D3D3 with their treatment effects in animal models of Alzheimer´s disease.

PMID:
26394756
PMCID:
PMC4585794
DOI:
10.1038/srep13222
[Indexed for MEDLINE]
Free PMC Article

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