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Immun Inflamm Dis. 2017 Sep;5(3):244-260. doi: 10.1002/iid3.160. Epub 2017 Apr 20.

Probiotic supplementation promotes a reduction in T-cell activation, an increase in Th17 frequencies, and a recovery of intestinal epithelium integrity and mitochondrial morphology in ART-treated HIV-1-positive patients.

Author information

1
Department of Public Health and Infectious Diseases, Azienda Policlinico Umberto I of Rome, Rome, Italy.
2
School of Biosciences, Veterinary Medicine University of Camerino, Matelica, Italy.
3
Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
4
Department of Therapeutic Research and Medicines Evaluation, Italian Institute of Health, Rome, Italy.
5
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
6
Section of Microbiology, Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
7
Department of Emergency Surgery-Emergency Endoscopic Unit, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
8
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.

Abstract

INTRODUCTION:

HIV infection is characterized by a persistent immune activation associated to a compromised gut barrier immunity and alterations in the profile of the fecal flora linked with the progression of inflammatory symptoms. The effects of high concentration multistrain probiotic (Vivomixx®, Viale del Policlinico 155, Rome, Italy in EU; Visbiome®, Dupont, Madison, Wisconsin in USA) on several aspects of intestinal immunity in ART-experienced HIV-1 patients was evaluated.

METHODS:

A sub-study of a longitudinal pilot study was performed in HIV-1 patients who received the probiotic supplement twice a day for 6 months (T6). T-cell activation and CD4+ and CD8+ T-cell subsets expressing IFNγ (Th1, Tc1) or IL-17A (Th17, Tc17) were stained by cytoflorimetric analysis. Histological and immunohistochemical analyses were performed on intestinal biopsies while enterocytes apoptosis index was determined by TUNEL assay.

RESULTS:

A reduction in the frequencies of CD4+ and CD8+ T-cell subsets, expressing CD38+ , HLA-DR+ , or both, and an increase in the percentage of Th17 cell subsets, especially those with central or effector memory phenotype, was recorded in the peripheral blood and in gut-associated lymphoid tissue (GALT) after probiotic intervention. Conversely, Tc1 and Tc17 levels remained substantially unchanged at T6, while Th1 cell subsets increase in the GALT. Probiotic supplementation was also associated to a recovery of the integrity of the gut epithelial barrier, a reduction of both intraepithelial lymphocytes density and enterocyte apoptosis and, an improvement of mitochondrial morphology sustained in part by a modulation of heat shock protein 60.

CONCLUSIONS:

These findings highlight the potential beneficial effects of probiotic supplementation for the reconstitution of physical and immunological integrity of the mucosal intestinal barrier in ART-treated HIV-1-positive patients.

KEYWORDS:

GALT; GUT; HIV-1; HSP60; IELs; T-cell activation; Tc1; Tc17; Th1; Th17; apoptosis; immunity; probiotics

PMID:
28474815
PMCID:
PMC5569369
DOI:
10.1002/iid3.160
[Indexed for MEDLINE]
Free PMC Article

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