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Predictable and precise template-free CRISPR editing of pathogenic variants.
Shen MW, Arbab M, Hsu JY, Worstell D, Culbertson SJ, Krabbe O, Cassa CA, Liu DR, Gifford DK, Sherwood RI. Shen MW, et al. Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. Epub 2018 Nov 7. Nature. 2018. PMID: 30405244 Free PMC article.
Following Cas9 cleavage, DNA repair without a donor template is generally considered stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that template-free Cas9 editing is predictable and capable of precise repair to …
Following Cas9 cleavage, DNA repair without a donor template is generally considered stochastic, heterogeneous and impractical beyond gene d …
Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations.
Grajcarek J, Monlong J, Nishinaka-Arai Y, Nakamura M, Nagai M, Matsuo S, Lougheed D, Sakurai H, Saito MK, Bourque G, Woltjen K. Grajcarek J, et al. Nat Commun. 2019 Oct 24;10(1):4856. doi: 10.1038/s41467-019-12829-8. Nat Commun. 2019. PMID: 31649251 Free PMC article.
Recently, the sequence context surrounding nuclease-induced double strand breaks (DSBs) has been shown to predict repair outcomes, for which H plays an important role. Here, we survey naturally occurring human deletion variants and identify that 11 million or 57% ar …
Recently, the sequence context surrounding nuclease-induced double strand breaks (DSBs) has been shown to predict repair outcomes, fo …
Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency.
Bermudez-Cabrera HC, Culbertson S, Barkal S, Holmes B, Shen MW, Zhang S, Gifford DK, Sherwood RI. Bermudez-Cabrera HC, et al. Nat Commun. 2021 Aug 25;12(1):5111. doi: 10.1038/s41467-021-25415-8. Nat Commun. 2021. PMID: 34433825 Free PMC article.
Mutational outcomes following CRISPR-Cas9-nuclease cutting in mammalian cells have recently been shown to be predictable and, in certain cases, skewed toward single genotypes. ...The ability to increase 1-bp insertion frequency adds another dimension to precise …
Mutational outcomes following CRISPR-Cas9-nuclease cutting in mammalian cells have recently been shown to be predictable and, …