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Antimicrob Agents Chemother. 2014 Aug;58(8):4961-5. doi: 10.1128/AAC.00125-14. Epub 2014 Jun 9.

Population structure of KPC-producing Klebsiella pneumoniae isolates from midwestern U.S. hospitals.

Author information

1
J. Craig Venter Institute, La Jolla, California, USA.
2
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
3
Department of Pathology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA.
4
Detroit Medical Center, Detroit, Michigan, USA.
5
Infectious Diseases Department, Cleveland Clinic, Cleveland, Ohio, USA.
6
Division of Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina, USA.
7
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
8
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA madams@jcvi.org Robert.Bonomo@va.gov.
9
J. Craig Venter Institute, La Jolla, California, USA madams@jcvi.org Robert.Bonomo@va.gov.

Abstract

Genome sequencing of carbapenem-resistant Klebsiella pneumoniae isolates from regional U.S. hospitals was used to characterize strain diversity and the bla(KPC) genetic context. A phylogeny based on core single-nucleotide variants (SNVs) supports a division of sequence type 258 (ST258) into two distinct groups. The primary differences between the groups are in the capsular polysaccharide locus (cps) and their plasmid contents. A strict association between clade and KPC variant was found. The bla(KPC) gene was found on variants of two plasmid backbones. This study indicates that highly similar K. pneumoniae subpopulations coexist within the same hospitals over time.

PMID:
24913165
PMCID:
PMC4136011
DOI:
10.1128/AAC.00125-14
[Indexed for MEDLINE]
Free PMC Article

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