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PLoS One. 2013 Oct 7;8(10):e76160. doi: 10.1371/journal.pone.0076160. eCollection 2013.

Expression and function of Allergin-1 on human primary mast cells.

Author information

1
Department of Immunology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Department of Nephrology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Tokyo, Japan.

Abstract

Mast cells (MC) play an important role in allergic and non-allergic immune responses. Activation of human MC is modulated by several cell surface inhibitory receptors, including recently identified Allergin-1 expressed on both human and mouse MC. Although Allergin-1 suppresses IgE-mediated, mast cell-dependent anaphylaxis in mice, the expression profile and function of Allergin-1 on human primary MC remains undetermined. Here, we established a seven-color flow cytometry method for assessing expression and function of a very small number of human primary MC. We show that Allergin-1S1, a splicing isoform of Allergin-1, is predominantly expressed on human primary MC in both bronchoalveolar lavage (BAL) fluid and nasal scratching specimens. Moreover, Allergin-1S1 inhibits IgE-mediated activation from human primary MC in BAL fluid. These results indicate that Allergin-1 on human primary MC exhibits similar characteristics as mouse Allergin-1 in the expression profile and function.

PMID:
24116093
PMCID:
PMC3792105
DOI:
10.1371/journal.pone.0076160
[Indexed for MEDLINE]
Free PMC Article

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