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Bone. 2014 Feb;59:189-98. doi: 10.1016/j.bone.2013.11.019. Epub 2013 Nov 27.

Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration.

Author information

1
Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
2
Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
3
Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea.
4
Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea. Electronic address: yongdae.kwon@gmail.com.
5
Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea. Electronic address: kwoni@khu.ac.kr.

Abstract

In this study we describe the generation and influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with growth and differentiation factor 5 (GDF-5). Prior to loading GDF-5, we characterized the release profiles from these hydrogels and tested their respective cell viability, differentiation and in vivo bone regeneration. The results from this testing indicated that GDF-5 was observed to release in a sustained manner from the HA hydrogels I-III. MTT and Live/Dead assays showed that the HA hydrogels I-III have good biocompatibility for use as scaffolds for bone tissue regeneration. In vitro cell tests showed a higher level of MC3T3-E1 cell proliferation and differentiation on HA hydrogels I-III than on HA hydrogel 0. Moreover, in vivo animal tests showed that the HA hydrogels I and III had a significant improvement on osteogenesis. Overall, our results suggest that the HA-based hydrogel is a good biomaterial to deliver osteogenic differentiation factors such as GDF-5, and GDF-5 can be useful as an effective alternative to aid new bone formation.

KEYWORDS:

Bone tissue regeneration; Growth and differentiation factor 5 (GDF-5); Hyaluronic acid; Hydrogel; Photopolymerization

PMID:
24291420
DOI:
10.1016/j.bone.2013.11.019
[Indexed for MEDLINE]

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