BLAST-XYPlot Viewer: A Tool for Performing BLAST in Whole-Genome Sequenced Bacteria/Archaea and Visualize Whole Results Simultaneously

G3 (Bethesda). 2018 Jul 2;8(7):2167-2172. doi: 10.1534/g3.118.200220.

Abstract

One of the most commonly used tools to compare protein or DNA sequences against databases is BLAST. We introduce a web tool that allows the performance of BLAST-searches of protein/DNA sequences in whole-genome sequenced bacteria/archaea, and displays a large amount of BLAST-results simultaneously. The circular bacterial replicons are projected as horizontal lines with fixed length of 360, representing the degrees of a circle. A coordinate system is created with length of the replicon along the x-axis and the number of replicon used on the y-axis. When a query sequence matches with a gene/protein of a particular replicon, the BLAST-results are depicted as an "x,y" position in a specially adapted plot. This tool allows the visualization of the results from the whole data to a particular gene/protein in real time with low computational resources.

Keywords: BLAST-XYPlot viewer; bacterial genomes; large scale BLAST; operon search.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / genetics
  • Bacteria / genetics
  • Computational Biology / methods*
  • Databases, Genetic
  • Genome, Archaeal
  • Genome, Bacterial
  • Genomics / methods
  • Software*
  • User-Computer Interface
  • Whole Genome Sequencing