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Virology. 2016 Oct;497:92-101. doi: 10.1016/j.virol.2016.07.006. Epub 2016 Jul 21.

Isolation of a monoclonal antibody that recognizes the origin binding domain of JCV, but not SV40, large T-antigen.

Author information

1
Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, 02111 MA, USA; Cell Essentials Inc., 75 Kneeland Street, Boston, MA 02111, USA.
2
Sackler Institute of Graduate Biomedical Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016-6481, USA.
3
Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
4
Institut de Recherches Cliniques de Montreal (IRCM), 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7; Department of Biochemistry and Molecular Medicine Universite de Montreal Montreal, Quebec, Canada.
5
Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, 02111 MA, USA.
6
Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA. Electronic address: Peter.Bullock@tufts.edu.

Abstract

Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodies raised against SV40 T-ag. Unfortunately, SV40 T-ag is also detected in humans and therefore there have been concerns about cross-reactivity. To address this issue, we have isolated a monoclonal antibody that binds to the JCV, but not the SV40, T-ag origin-binding domain (OBD). Furthermore, the region on the surface of the JCV T-ag OBD that is recognized by the "anti-JCV OBD mAb" has been mapped. We also demonstrate that the "anti-JCV OBD mAb" will be a useful reagent for standard techniques (e.g., Westerns blots and ELISAs). Finally, we note that additional monoclonal Abs that are specific for the T-ags encoded by the other human polyomaviruses could be generated by adopting the approach described herein.

KEYWORDS:

JCV; Monoclonal antibody; Polyomavirus

PMID:
27433780
DOI:
10.1016/j.virol.2016.07.006
[Indexed for MEDLINE]
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