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Nat Methods. 2016 Jan;13(1):59-62. doi: 10.1038/nmeth.3667. Epub 2015 Nov 30.

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.

Author information

1
Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
2
Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Hamburg, Germany.
3
Lawrence Berkeley National Laboratory, Berkeley, California, USA.
4
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
5
Institute für Biologie, Humboldt University of Berlin, Berlin, Germany.
6
Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
7
Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.

Abstract

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).

PMID:
26619013
PMCID:
PMC4890631
DOI:
10.1038/nmeth.3667
[Indexed for MEDLINE]
Free PMC Article

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