Format

Send to

Choose Destination

See 1 citation:

Methods Mol Biol. 2015;1327:209-19. doi: 10.1007/978-1-4939-2842-2_15.

A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae.

Author information

1
Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA.
2
Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA. shall@syr.edu.

Abstract

The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.

KEYWORDS:

Caenorhabditis elegans; Dauer; Diapause; Egg white plate; Pheromone; Postdauer; Stress

PMID:
26423977
DOI:
10.1007/978-1-4939-2842-2_15
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center