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J Cell Sci. 2018 May 4;131(9). pii: jcs216176. doi: 10.1242/jcs.216176.

Adenylyl cyclase A mRNA localized at the back of cells is actively translated in live chemotaxing Dictyostelium.

Wang W1, Chen S1,2,3, Das S1,3, Losert W3, Parent CA4,2,3.

Author information

1
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA.
2
Department of Pharmacology, Michigan Medicine, Ann Arbor, MI 48109, USA.
3
Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.
4
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA parentc@umich.edu.

Abstract

Dictyostelium discoideum cells transport adenylyl cyclase A (ACA)-containing vesicles to the back of polarized cells to relay exogenous cAMP signals during chemotaxis. Fluorescence in situ hybridization (FISH) experiments showed that ACA mRNA is also asymmetrically distributed at the back of polarized cells. By using the MS2 bacteriophage system, we now visualize the distribution of ACA mRNA in live chemotaxing cells. We found that the ACA mRNA localization is not dependent on the translation of the protein product and requires multiple cis-acting elements within the ACA-coding sequence. We show that ACA mRNA is associated with actively translating ribosomes and is transported along microtubules towards the back of cells. By monitoring the recovery of ACA-YFP after photobleaching, we observed that local translation of ACA-YFP occurs at the back of cells. These data represent a novel functional role for localized translation in the relay of chemotactic signals during chemotaxis.

KEYWORDS:

Cell migration; Dictyostelium; Live imaging; Translation; mRNA

PMID:
29618632
PMCID:
PMC5992586
DOI:
10.1242/jcs.216176
[Indexed for MEDLINE]
Free PMC Article

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