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Cell Host Microbe. 2016 Apr 13;19(4):529-40. doi: 10.1016/j.chom.2016.03.005.

Th17 Cells Are Preferentially Infected Very Early after Vaginal Transmission of SIV in Macaques.

Author information

1
Department of Cellular and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
2
Tulane National Primate Research Center, Tulane University School of Medicine, Covington, LA 70433, USA.
3
Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
4
Department of Cellular and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: thope@northwestern.edu.

Abstract

The difficulty in detecting rare infected cells immediately after mucosal HIV transmission has hindered our understanding of the initial cells targeted by the virus. Working with the macaque simian immunodeficiency virus (SIV) vaginal challenge model, we developed methodology to identify discrete foci of SIV (mac239) infection 48 hr after vaginal inoculation. We find infectious foci throughout the reproductive tract, from labia to ovary. Phenotyping infected cells reveals that SIV has a significant bias for infection of CCR6+ CD4+ T cells. SIV-infected cells expressed the transcriptional regulator RORγt, confirming that the initial target cells are specifically of the Th17 lineage. Furthermore, we detect host responses to infection, as evidenced by apoptosis, cell lysis, and phagocytosis of infected cells. Thus, our analysis identifies Th17-lineage CCR6+ CD4+ T cells as primary targets of SIV during vaginal transmission. This opens new opportunities for interventions to protect these cells and prevent HIV transmission.

PMID:
27078070
PMCID:
PMC4841252
DOI:
10.1016/j.chom.2016.03.005
[Indexed for MEDLINE]
Free PMC Article

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