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Stem Cell Reports. 2014 Jun 6;3(1):185-203. doi: 10.1016/j.stemcr.2014.05.002. eCollection 2014 Jul 8.

A human pluripotent stem cell surface N-glycoproteome resource reveals markers, extracellular epitopes, and drug targets.

Author information

1
Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SAR ; National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA ; Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
2
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
3
National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
4
Department of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, Switzerland.
5
Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
6
Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SAR ; Department of Pediatrics & Adolescent Medicine, Hong Kong University, Hong Kong, SAR.
7
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA ; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA ; Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53226, USA.

Abstract

Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.

PMID:
25068131
PMCID:
PMC4110789
DOI:
10.1016/j.stemcr.2014.05.002
[Indexed for MEDLINE]
Free PMC Article

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