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  • The following term was not found in PubMed: b-D-galactopyranosyl-D-xyloses.
Bioorg Med Chem. 2007 Jul 15;15(14):4836-40. Epub 2007 May 6.

Optimizing the enzymatic synthesis of beta-D-galactopyranosyl-D-xyloses for their use in the evaluation of lactase activity in vivo.

Author information

1
Departamento de Química Orgánica Biológica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.

Abstract

Disaccharides 2-O-, 3-O-, and 4-O-beta-D-galactopyranosyl-D-xyloses (2, 3, and 1, respectively) were obtained by beta-galactosidase-catalyzed reactions for their use in the evaluation of intestinal lactase activity in vivo. Their administration to suckling rats followed by determination of the derived D-xylose in the urine and measurement of lactase activity in intestinal homogenates showed 1 to be the most suitable disaccharide for a potential test of the deficiency of intestinal lactase. The synthesis of 1 was further studied by evaluating the effect of different variables on the yield and regioselectivity of the enzymatic galactosylation, and the purification process was optimized.

PMID:
17512743
DOI:
10.1016/j.bmc.2007.04.067
[Indexed for MEDLINE]

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