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Sci Rep. 2017 Mar 27;7(1):436. doi: 10.1038/s41598-017-00594-x.

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing.

Liu M1,2, Luo G1,2, Wang Y1,2, Xu R1,2, Wang Y1,2, He W1,2, Tan J1,2, Xing M3,4,5,6, Wu J7,8,9.

Author information

1
Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
2
Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
3
Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. malcolm.xing@umanitoba.ca.
4
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.
5
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.
6
Children's Hospital Research Institute of Manitoba, Winnipeg, Canada. malcolm.xing@umanitoba.ca.
7
Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. editorinchief@burninchina.com.
8
Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China. editorinchief@burninchina.com.
9
Department of Plastic and Burn Surgery, the First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, China. editorinchief@burninchina.com.

Abstract

An ideal wound dressing can both promote wound healing and prevent bacterial infection. Here, we report a potential dressing prepared by incorporating an optimized concentration of silver nanoparticles (AgNPs) into the microfibers of a natural eggshell membrane (EM) using environmentally friendly and mussel-inspired dopamine. Briefly, acid-treated EM was used as a porous membrane for polydopamine-reduced AgNPs synthesis. To obtain the optimal cytocompatible silver concentration, cellular attachment and MTT assay were performed with different concentrations of AgNPs. The morphology of the EM and AgNPs was confirmed by scanning electronic microscopy, scanning transmission electronic microscopy and Fourier transform infrared spectroscopy. The synthesized EM/AgNPs exhibited steady and safe AgNPs release, which was further tested for antibacterial activity against Escherichia coli and Staphylococcus aureus by disc diffusion method and bacterial suspension assay. Finally, in a murine full-thickness skin wound model, we found that EM/AgNPs could promote re-epithelialization, granulation tissue formation and wound healing via enhancing cell proliferation, as demonstrated by the expression of proliferating cell nuclear antigen (PCNA), and controlling inflammation response, as demonstrated by the expression of interleukin-1β (IL-1β). These findings suggest that EM/AgNPs may have a promising application in wound management.

PMID:
28348388
PMCID:
PMC5428678
DOI:
10.1038/s41598-017-00594-x
[Indexed for MEDLINE]
Free PMC Article

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