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Biosens Bioelectron. 2018 Oct 15;117:104-111. doi: 10.1016/j.bios.2018.05.043. Epub 2018 May 25.

Nano-biosensor for highly sensitive detection of HER2 positive breast cancer.

Author information

1
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
2
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran. Electronic address: ghaffarinejad@iust.ac.ir.
3
Nanobioengineering Division, Department of Nanotechnology, School of New Technologies, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran.
4
Genetics Department, Breast Cancer Research Center (BCRC), Motamed Cancer Institute, ACECR, Tehran, Iran. Electronic address: kmajidzadeh@razi.tums.ac.ir.
5
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
6
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4. Electronic address: amir.sanatinezhad@ucalgary.ca.

Abstract

Nanocomposite materials have provided a wide range of conductivity, sensitivity, selectivity and linear response for electrochemical biosensors. However, the detection of rare cells at single cell level requires a new class of nanocomposite-coated electrodes with exceptional sensitivity and specificity. We recently developed a construct of gold nanoparticle-grafted functionalized graphene and nanostructured polyaniline (PANI) for high-performance biosensing within a very wide linear response and selective performance. Further, replacing the expensive gold nanoparticles with low-cost silver nanoparticles as well as optimizing the nanocomposite synthesis and functionalization protocols on the electrode surface in this work enabled us to develop ultrasensitive nanocomposites for label-free detection of breast cancer cells. The sensor presented a fast response time of 30 min within a dynamic range of 10 - 5 × 106 cells mL-1 and with a detection limit of 2 cells mL-1 for the detection of SK-BR3 breast cancer cell. The nano-biosensor, for the first time, demonstrated a high efficiency of > 90% for the label-free detection of cancer cells in whole blood sample without any need for sample preparation and cell staining. The results demonstrated that the optimized nanocomposite developed in this work is a promising nanomaterial for electrochemical biosensing and with the potential applications in electro-catalysis and super-capacitances.

KEYWORDS:

Breast cancer; Electrochemical biosensing; Nanostructured polyaniline nanocomposite; Single cell detection

PMID:
29890392
DOI:
10.1016/j.bios.2018.05.043
[Indexed for MEDLINE]

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