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Sci Rep. 2016 May 24;6:26714. doi: 10.1038/srep26714.

Nano-SPRi Aptasensor for the Detection of Progesterone in Buffer.

Author information

1
Department of Nanoscience, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
2
Molecular Core Lab, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.
3
Center for Biotechnology, Genomics, and Health Research, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
4
HORIBA Scientific, Edison, NJ 08820, USA.

Abstract

Progesterone is a steroid hormone that plays a central role in the female reproductive processes such as ovulation and pregnancy with possible effects on other organs as well. The measurement of progesterone levels in bodily fluids can assist in early pregnancy diagnosis and can provide insight for other reproductive functions. In this work, the detection of progesterone was examined by integrating novel aptamer development with a nanoEnhanced surface plasmon resonance imaging sensor. First, we developed X-aptamers and selected them for binding to progesterone. Then, we took advantage of the multi-array feature of SPRi to develop an optimized biosensor capable of simultaneously screening the 9 X-aptamers developed to determine the binding capabilities of each aptamer. The sensor surface design conditions were further optimized for the sandwich assay, which employed nanoEnhancers (NIR-streptavidin coated quantum dots) for ultrasensitive detection of progesterone molecules. The assay designed was examined over a concentration range of 1.575 ng/mL to 126 μg/mL resulting in a limit of detection (LOD) of 1.575 ng/mL (5 nM) in phosphate buffer.

PMID:
27216648
PMCID:
PMC4877642
DOI:
10.1038/srep26714
[Indexed for MEDLINE]
Free PMC Article

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