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Elife. 2015 Sep 15;4:e08649. doi: 10.7554/eLife.08649.

NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans.

Author information

1
Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
2
Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, United States.
3
Institut Génétique Biologie Moléculaire Ceasllulaire, Faculté de médecine, Université de Strasbourg, Strasbourg, France.
4
Institut Clinique de la Souris, Illkirch-Graffenstaden, France.

Abstract

Non-centrosomal microtubule arrays assemble in differentiated tissues to perform mechanical and transport-based functions. In this study, we identify Caenorhabditis elegans NOCA-1 as a protein with homology to vertebrate ninein. NOCA-1 contributes to the assembly of non-centrosomal microtubule arrays in multiple tissues. In the larval epidermis, NOCA-1 functions redundantly with the minus end protection factor Patronin/PTRN-1 to assemble a circumferential microtubule array essential for worm growth and morphogenesis. Controlled degradation of a γ-tubulin complex subunit in this tissue revealed that γ-tubulin acts with NOCA-1 in parallel to Patronin/PTRN-1. In the germline, NOCA-1 and γ-tubulin co-localize at the cell surface, and inhibiting either leads to a microtubule assembly defect. γ-tubulin targets independently of NOCA-1, but NOCA-1 targeting requires γ-tubulin when a non-essential putatively palmitoylated cysteine is mutated. These results show that NOCA-1 acts with γ-tubulin to assemble non-centrosomal arrays in multiple tissues and highlight functional overlap between the ninein and Patronin protein families.

KEYWORDS:

C. elegans; NOCA-1; PTRN-1; Patronin; cell biology; developmental biology; ninein; non-centrosomal microtubule array; stem cells

PMID:
26371552
PMCID:
PMC4608005
DOI:
10.7554/eLife.08649
[Indexed for MEDLINE]
Free PMC Article

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