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J Cell Sci. 2019 Aug 22;132(16). pii: jcs230177. doi: 10.1242/jcs.230177.

Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons.

Author information

1
DFG Emmy Noether Group 'Neuronal Protein Transport', Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
2
Institute for Synaptic Physiology, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
3
Institute of Structural Neurobiology, Center for Molecular Neurobiology Hamburg, ZMNH, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
4
Institute of Clinical Neuroanatomy, Faculty of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany.
5
Center for Proteomics, Erasmus MC, 3000 CA Rotterdam, The Netherlands.
6
Cell Biology and Physiology Center, National Heart, Lung Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA.
7
Department of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
8
DFG Emmy Noether Group 'Neuronal Protein Transport', Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany marina.mikhaylova@zmnh.uni-hamburg.de.

Abstract

The spine apparatus (SA) is an endoplasmic reticulum-related organelle that is present in a subset of dendritic spines in cortical and pyramidal neurons, and plays an important role in Ca2+ homeostasis and dendritic spine plasticity. The protein synaptopodin is essential for the formation of the SA and is widely used as a maker for this organelle. However, it is still unclear which factors contribute to its localization at selected synapses, and how it triggers local SA formation. In this study, we characterized development, localization and mobility of synaptopodin clusters in hippocampal primary neurons, as well as the molecular dynamics within these clusters. Interestingly, synaptopodin at the shaft-associated clusters is less dynamic than at spinous clusters. We identify the actin-based motor proteins myosin V (herein referring to both the myosin Va and Vb forms) and VI as novel interaction partners of synaptopodin, and demonstrate that myosin V is important for the formation and/or maintenance of the SA. We found no evidence of active microtubule-based transport of synaptopodin. Instead, new clusters emerge inside spines, which we interpret as the SA being assembled on-site.

KEYWORDS:

Dendritic spines; F-actin; Myosin; Spine apparatus; Synaptopodin

PMID:
31371487
DOI:
10.1242/jcs.230177
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Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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