Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Th1 subtype response in vitro

Liping Ye; Yanlong Jiang; Guilian Yang; Wentao Yang; Jingtao Hu; Yulin Cui; Chunwei Shi; Jing Liu; Chunfeng Wang

doi:10.1016/j.micpath.2017.07.015

Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Th1 subtype response in vitro

Microb Pathog. 2017 Sep:110:325-334. doi: 10.1016/j.micpath.2017.07.015. Epub 2017 Jul 12.

Authors

Liping Ye¹, Yanlong Jiang¹, Guilian Yang¹, Wentao Yang¹, Jingtao Hu¹, Yulin Cui¹, Chunwei Shi¹, Jing Liu¹, Chunfeng Wang²

Affiliations

¹ College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
² College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.

PMID: 28710013
DOI: 10.1016/j.micpath.2017.07.015

Abstract

Rotavirus (RV) infection causes acute, watery dehydrating diarrhea and even death in infants and other young animals, resulting in a severe economic burden; however, little is known about the innate immune mechanisms associated with RV infection. Dendritic cells (DCs), which are professional antigen-presenting cells (APCs), serve as a bridge connecting the innate and adaptive immune system. In this study, the interaction between murine bone marrow-derived DCs (BMDCs) and porcine rotavirus (PRV) was investigated in vitro. Upon stimulation, the expression levels of MHC-II, CD40, CD80, CD86 and CD83 in BMDCs increased in a time-dependent manner, indicating activation and maturation by PRV. In addition, up-regulated Toll-like receptor 2 (TLR2), TLR3 and NF-κB increased the production of interleukin-12 and interferon-γ. The PRV-stimulated BMDCs also showed increased stimulatory capacity in mixed lymphocyte reactions and promoted the Th1 subtype response.

Keywords: Activation; Bone marrow-derived DCs (BMDCs); Rotavirus.

MeSH terms

Adaptive Immunity
Animals
Antigen-Presenting Cells / immunology
Antigen-Presenting Cells / virology
Antigens, CD / metabolism
B7-1 Antigen / metabolism
B7-2 Antigen / metabolism
Bone Marrow / immunology*
CD40 Antigens / metabolism
CD83 Antigen
Cytokines / genetics
Cytokines / metabolism
Dendritic Cells / cytology
Dendritic Cells / immunology*
Dendritic Cells / metabolism
Dendritic Cells / virology*
Gene Expression Regulation
Genes, MHC Class II
Host-Pathogen Interactions / immunology
Immunity, Innate
Immunity, Mucosal
Immunoglobulins / metabolism
Interferon-gamma / metabolism
Interleukin-12 / metabolism
Male
Membrane Glycoproteins / metabolism
Mice
Mice, Inbred BALB C
NF-kappa B / metabolism
Rotavirus / immunology*
Rotavirus / pathogenicity
Rotavirus Infections / immunology*
Swine
Th1 Cells / immunology*
Toll-Like Receptor 2 / genetics
Toll-Like Receptor 2 / metabolism
Toll-Like Receptor 3 / genetics
Toll-Like Receptor 3 / metabolism
Up-Regulation

Substances

Antigens, CD
B7-1 Antigen
B7-2 Antigen
CD40 Antigens
Cytokines
Immunoglobulins
Membrane Glycoproteins
NF-kappa B
TLR3 protein, mouse
Toll-Like Receptor 2
Toll-Like Receptor 3
Interleukin-12
Interferon-gamma