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J Proteomics. 2015 Oct 14;128:105-12. doi: 10.1016/j.jprot.2015.07.028. Epub 2015 Jul 29.

Multi-omics profiling reveals that eating difficulties developed consecutively to artificial nutrition in the neonatal period are associated to specific saliva composition.

Author information

1
CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France. Electronic address: Martine.Morzel@dijon.inra.fr.
2
CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
3
IFR 100 Santé-STIC, CLIPP (Clinical and Innovation Proteomic Platform), F-21000 Dijon, France.
4
INRA, UMR 1331 TOXALIM, Research Centre in Food Toxicology, F-31027 Toulouse, France.
5
CHU Montpellier, Institute of Research in Biotherapy, Hôpital Saint Eloi, Laboratory of Biochemistry and Clinical Proteomics, INSERM U1040, F-34000 Montpellier France.
6
INSERM, CIC 1407, Hospices Civils de Lyon, CNRS, UMR5558, Université de Lyon, F-69000 Lyon, France.
7
Université de Lyon, Claude Bernard Lyon 1, Faculté de Médecine Lyon-Est, INSERM U1060, CarMeN laboratory, Hospices Civils de Lyon, F-69003 Lyon, France.

Abstract

Prolonged enteral or parenteral nutrition in neonatal periods sometimes results in eating difficulties persisting for years, with reduced food intake through the oral route and thereby reduced stimulation of the oral cavity. Aiming at describing the consequences on oral physiology, saliva of 21 children with eating difficulties (ED) was compared to that of 23 healthy controls, using various omics and targeted methods. Overall, despite heterogeneity within the groups (age, medication etc.), the three spectral methods (MALDI-TOF, SELDI-TOF, (1)H NMR) allowed discriminating ED and controls, confirming that oral stimulation by food intake plays a role in shaping the composition of saliva. Saliva of ED patients exhibited a lower antioxidant status and lower levels of the salivary protease inhibitors cystatins. Other discriminant features (IgA1, dimethylamine) may relate to modified oral and/or intestinal microbial ecology. Finally, salivary profiles of ED patients were partly comparable to those of subjects with exacerbated gustatory sensitivities, in particular with reduced abundance of cystatin SN and higher abundance of zinc-alpha-2-glycoprotein. Whether this translates taste hypersensitivity and contributes to the eating difficulties deserves further attention.

KEYWORDS:

Children; Feeding difficulties; Profiling; Saliva; Tube feeding

PMID:
26232109
DOI:
10.1016/j.jprot.2015.07.028
[Indexed for MEDLINE]

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