Format

Send to

Choose Destination

See 1 citation found by title matching your search:

PLoS One. 2017 Sep 22;12(9):e0185434. doi: 10.1371/journal.pone.0185434. eCollection 2017.

Molecular detection of Borrelia burgdorferi sensu lato - An analytical comparison of real-time PCR protocols from five different Scandinavian laboratories.

Author information

1
Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
2
Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
3
German National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
4
Clinical Microbiology, Laboratory Medicine, Region Jönköping County, Sweden.
5
Faculty of Engineering and Science, Department of Natural Sciences, University of Agder, Kristiansand, Norway.
6
Research Unit, Hospital of Southern Norway Trust, Kristiansand, Norway.
7
Division of Infectious Disease Control, Department of Virology, Norwegian Institute of Public Health, Oslo, Norway.
8
Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
9
Department of Medical Microbiology, Hospital of Southern Norway Trust, Kristiansand, Norway.
10
Division of Medicine, Skånevård Kryh, Region Skåne, Sweden.
11
Division of Laboratory Medicine, Department of Clinical Microbiology, Lund, Sweden.
12
Karolinska University Laboratory, Stockholm, Sweden.
13
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Abstract

INTRODUCTION:

Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today is based on the patient´s medical history, clinical presentation and laboratory findings. The laboratory diagnostics are mainly based on antibody detection, but in certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement.

AIM:

The purpose of this study was to evaluate the analytical sensitivity, analytical specificity and concordance of eight different real-time PCR methods at five laboratories in Sweden, Norway and Denmark.

METHOD:

Each participating laboratory was asked to analyse three different sets of samples (reference panels; all blinded) i) cDNA extracted and transcribed from water spiked with cultured Borrelia strains, ii) cerebrospinal fluid spiked with cultured Borrelia strains, and iii) DNA dilution series extracted from cultured Borrelia and relapsing fever strains. The results and the method descriptions of each laboratory were systematically evaluated.

RESULTS AND CONCLUSIONS:

The analytical sensitivities and the concordance between the eight protocols were in general high. The concordance was especially high between the protocols using 16S rRNA as the target gene, however, this concordance was mainly related to cDNA as the type of template. When comparing cDNA and DNA as the type of template the analytical sensitivity was in general higher for the protocols using DNA as template regardless of the use of target gene. The analytical specificity for all eight protocols was high. However, some protocols were not able to detect Borrelia spielmanii, Borrelia lusitaniae or Borrelia japonica.

PMID:
28937997
PMCID:
PMC5609768
DOI:
10.1371/journal.pone.0185434
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center