Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Biochim Biophys Acta. 2011 Dec;1810(12):1150-9. doi: 10.1016/j.bbagen.2011.09.016. Epub 2011 Oct 4.

Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1.

Author information

1
Department of Microbiology and Immunochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510, Japan.

Abstract

BACKGROUND:

Scavenger receptors are generally expressed in macrophages and vascular endothelial cells and some scavenger receptors are thought to contribute to the development of atherosclerosis.

METHODS:

We cloned the cDNA of a zebrafish CL-P1 (collectin placenta 1) and performed a knockdown study using its antisense morpholino oligonucleotides (MO).

RESULTS:

Zebrafish CL-P1 (zCL-P1) is 51% identical to human CL-P1 in its amino acid sequence. Microbes and OxLDL bound to zCL-P1 cDNA transfected cells. zCL-P1 mRNA expression gradually increased after 6hours post-fertilization (hpf), reached its highest level at 24hpf, and then decreased, which is similar to the gene expression pattern of Tie-2. The knockdown of zCL-P1 led to an increase in the number of zebrafish embryos with severe morphological abnormalities such as short body lengths and defects in the dorsal aorta at 48hpf. Simultaneous injection of both MO and synthetic zCL-P1 or zVEGF mRNA rescued the abnormal phenotype.

CONCLUSIONS:

In vivo knockdown study shows that zCL-P1 is implicated in vasculogenesis and those of our in vitro study support its role as a scavenger receptor.

GENERAL SIGNIFICANCE:

These results suggest that zCL-P1 might be essential for vasculogenesis during the early embryonic phase in bone fish.

PMID:
22001438
DOI:
10.1016/j.bbagen.2011.09.016
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center