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Homeopathy. 2014 Oct;103(4):264-74. doi: 10.1016/j.homp.2014.08.006. Epub 2014 Oct 3.

Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH.

Author information

1
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil.
2
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil; Laboratory of Veterinary Pathology, University of Santo Amaro, São Paulo, Brazil.
3
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil; Laboratory of Immunology, Federal University of São Paulo, São Paulo, Brazil.
4
Veterinary and Zootechny Faculty, University of São Paulo, Brazil.
5
Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
6
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil; Laboratory of Veterinary Pathology, University of Santo Amaro, São Paulo, Brazil. Electronic address: leonibonamin@gmail.com.

Abstract

BACKGROUND:

Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. The modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.

METHODS:

We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 × 10(6)Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes present in the peritoneal washing fluid and spleen were analyzed by flow cytometry and histopathology, with histometry of the subcutaneous primary lesion, local lymph node and spleen. Immunohistochemistry was performed to quantify CD3 (T lymphocyte), CD45RA (B lymphocyte) and CD11b (phagocytes) positive cells.

RESULTS:

In treated mice, during the acute phase, there was significant increase of the macroscopic lesion, associated to inflammatory edema, as well increase in the number of free amastigotes and B lymphocytes inside the lesion. Increase of B lymphocytes (predominantly B-2 cells) was also seen in the local lymph node, spleen and peritoneum. In the chronic phase, the inflammatory process in the infection focus was reduced, with reduced phagocyte migration and peritoneal increase of B-1a cells (precursors of B-2 immunoglobulin producers cells) and T CD8+ cells.

CONCLUSION:

The treatment of mice with Antimonium crudum 30cH induced a predominantly B cell pattern of immune response in Leishmania (L.) amazonensis experimental infection, alongside the increase of free amastigote forms number in the infection site. The clinical significance of this study is discussed, further studies are suggested.

KEYWORDS:

Antimonium crudum; Homeopathy; Leishmania (L.) amazonensis; Murine leishmaniasis

PMID:
25439043
DOI:
10.1016/j.homp.2014.08.006
[Indexed for MEDLINE]

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