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Fertil Steril. 2002 Nov;78(5):1046-8.

Mitochondrial deletions in luteinized granulosa cells as a function of age in women undergoing in vitro fertilization.

Author information

1
Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Endocrinology and Infertility, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA. seiferdb@umdnj.edu

Abstract

OBJECTIVE:

To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF.

DESIGN:

Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA.

SETTING:

University-based fertility clinic.

PATIENT(S):

Twenty-four women divided equally between two groups: </=34 years old and >/=38 years old.

INTERVENTION(S):

Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration.

MAIN OUTCOME MEASURE(S):

Presence or absence of the 4977-bp deletion in human mtDNA.

RESULT(S):

Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment.

CONCLUSION(S):

These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women </=34 years.

PMID:
12413991
DOI:
10.1016/s0015-0282(02)04214-0
[Indexed for MEDLINE]

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