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Sci Rep. 2015 Mar 13;5:9073. doi: 10.1038/srep09073.

MitoCeption as a new tool to assess the effects of mesenchymal stem/stromal cell mitochondria on cancer cell metabolism and function.

Author information

1
1] IRMB CHU Saint Eloi, 80 rue Augustin Fliche, 34295 Montpellier cedex 5, University of Montpellier, France [2] Inserm U1183,CNRS UMR 5535/IFR122, 1919 route de Mende, 34293 Montpellier Cedex 5, University of Montpellier, France.
2
Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535/IFR122, 1919 route de Mende, 34293 Montpellier Cedex 5, University of Montpellier, France.
3
1] Univ Grenoble Alpes, 38000 Grenoble, France [2] Inserm, U823 and U836, 38000 Grenoble, France [3] Grenoble University hospital, Institut of Biologie and Pathology, 38000 Grenoble, France.
4
1] Univ Grenoble Alpes, 38000 Grenoble, France [2] Inserm, U823 and U836, 38000 Grenoble, France [3] French blood company/Grenoble University hospital, Cell Therapy Unit, 38000 Grenoble, France.
5
1] IRMB CHU Saint Eloi, 80 rue Augustin Fliche, 34295 Montpellier cedex 5, University of Montpellier, France [2] Inserm U1051, Montpellier, France.
6
1] IRMB CHU Saint Eloi, 80 rue Augustin Fliche, 34295 Montpellier cedex 5, University of Montpellier, France [2] Inserm U1183,CNRS UMR 5535/IFR122, 1919 route de Mende, 34293 Montpellier Cedex 5, University of Montpellier, France [3] Department for Biotherapy at CHU Lapeyronie University Hospital, Montpellier, France.

Abstract

Mitochondrial activity is central to tissue homeostasis. Mitochondria dysfunction constitutes a hallmark of many genetic diseases and plays a key role in tumor progression. The essential role of mitochondria, added to their recently documented capacity to transfer from cell to cell, obviously contributes to their current interest. However, determining the proper role of mitochondria in defined biological contexts was hampered by the lack of suitable experimental tools. We designed a protocol (MitoCeption) to directly and quantitatively transfer mitochondria, isolated from cell type A, to recipient cell type B. We validated and quantified the effective mitochondria transfer by imaging, fluorescence-activated cell sorting (FACS) and mitochondrial DNA analysis. We show that the transfer of minute amounts of mesenchymal stem/stromal cell (MSC) mitochondria to cancer cells, a process otherwise occurring naturally in coculture, results in cancer cell enhanced oxidative phosphorylation (OXPHOS) activity and favors cancer cell proliferation and invasion. The MitoCeption technique, which can be applied to different cell systems, will therefore be a method of choice to analyze the metabolic modifications induced by exogenous mitochondria in host cells.

PMID:
25766410
PMCID:
PMC4358056
DOI:
10.1038/srep09073
[Indexed for MEDLINE]
Free PMC Article

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