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See 1 citation in 2014 by Hawkins NA and Kearney JA and McCollom CE and Miller AR:

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Genes Brain Behav. 2014 Feb;13(2):163-72. doi: 10.1111/gbb.12099. Epub 2013 Nov 14.

Mapping genetic modifiers of survival in a mouse model of Dravet syndrome.

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  • 1Department of Medicine, Vanderbilt University, Nashville, TN, USA.

Abstract

Epilepsy is a common neurological disorder affecting approximately 1% of the population. Mutations in voltage-gated sodium channels are responsible for several monogenic epilepsy syndromes. More than 800 mutations in the voltage-gated sodium channel SCN1A have been reported in patients with generalized epilepsy with febrile seizures plus and Dravet syndrome. Heterozygous loss-of-function mutations in SCN1A result in Dravet syndrome, a severe infant-onset epileptic encephalopathy characterized by intractable seizures, developmental delays and increased mortality. A common feature of monogenic epilepsies is variable expressivity among individuals with the same mutation, suggesting that genetic modifiers may influence clinical severity. Mice with heterozygous deletion of Scn1a (Scn1a(+/-) ) model a number of Dravet syndrome features, including spontaneous seizures and premature lethality. Phenotype severity in Scn1a(+/-) mice is strongly dependent on strain background. On the 129S6/SvEvTac strain Scn1a(+/-) mice exhibit no overt phenotype, whereas on the (C57BL/6J × 129S6/SvEvTac)F1 strain Scn1a(+/-) mice exhibit spontaneous seizures and early lethality. To systematically identify loci that influence premature lethality in Scn1a(+/-) mice, we performed genome scans on reciprocal backcrosses. Quantitative trait locus mapping revealed modifier loci on mouse chromosomes 5, 7, 8 and 11. RNA-seq analysis of strain-dependent gene expression, regulation and coding sequence variation provided a list of potential functional candidate genes at each locus. Identification of modifier genes that influence survival in Scn1a(+/-) mice will improve our understanding of the pathophysiology of Dravet syndrome and may suggest novel therapeutic strategies for improved treatment of human patients.

KEYWORDS:

Dravet syndrome; RNA-seq; epilepsy; epileptic encephalopathy; mouse model; seizures; severe myoclonic epilepsy of infancy; transcriptomics; voltage-gated ion channels; voltage-gated sodium channels

PMID:
24152123
PMCID:
PMC3930200
DOI:
10.1111/gbb.12099
[PubMed - indexed for MEDLINE]
Free PMC Article
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