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SLAS Technol. 2017 Aug;22(4):425-430. doi: 10.1177/2211068216680208. Epub 2016 Nov 19.

Microfluidic Tissue Mesodissection in Molecular Cancer Diagnostics.

Author information

1
1 Electronics Organization, GE Global Research Center, Niskayuna, NY, USA.
2
2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
3
3 NeuroInDx, Inc., Torrance, CA, USA.

Abstract

We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive. We demonstrate a microfluidic tool for rapid extraction of histological regions of interest from formalin-fixed paraffin-embedded tissue, which uses a simple and automated method that is compatible with most downstream enzymatic reactions, including protocols used for next-generation DNA sequencing.

KEYWORDS:

and genomics; clinical automation; microfluidics; molecular biology; robotics and instrumentation

PMID:
27864340
DOI:
10.1177/2211068216680208
[Indexed for MEDLINE]

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