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Epilepsia. 2018 Jul;59(7):1455-1468. doi: 10.1111/epi.14441. Epub 2018 Jun 11.

Mechanisms of increased hippocampal excitability in the Mashl+/- mouse model of Na+ /K+ -ATPase dysfunction.

Author information

1
Division of Pediatric Neurology, Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
2
Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA.
3
Durham Veterans Affairs Medical Center, Durham, NC, USA.
4
Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC, USA.
5
Veterans Affairs Mid-Atlantic Region Mental Illness Research, Education, and Clinical Center, Durham, NC, USA.
6
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA.

Abstract

OBJECTIVE:

Na+ /K+ -ATPase dysfunction, primary (mutation) or secondary (energy crisis, neurodegenerative disease) increases neuronal excitability in the brain. To evaluate the mechanisms underlying such increased excitability we studied mice carrying the D801N mutation, the most common mutation causing human disease, specifically alternating hemiplegia of childhood (AHC) including epilepsy. Because the gene is expressed in all neurons, particularly γ-aminobutyric acid (GABA)ergic interneurons, we hypothesized that the pathophysiology would involve both pyramidal cells and interneurons and that fast-spiking interneurons, which have increased firing rates, would be most vulnerable.

METHODS:

We performed extracellular recordings, as well as whole-cell patch clamp recordings from pyramidal cells and interneurons, in the CA1 region on hippocampal slices. We also performed immunohistochemistry from hippocampal sections to count CA1 pyramidal cells as well as parvalbumin-positive interneurons. In addition, we performed video-electroencephalography (EEG) recordings from the dorsal hippocampal CA1 region.

RESULTS:

We observed that juvenile knock-in mice carrying the above mutation reproduce the human phenotype of AHC. We then demonstrated in the CA1 region of these mice the following findings as compared to wild type: (1) Increased number of spikes evoked by electrical stimulation of Schaffer collaterals; (2) equalization by bicuculline of the number of spikes induced by Schaffer collateral stimulation; (3) reduced miniature, spontaneous, and evoked inhibitory postsynaptic currents, but no change in excitatory postsynaptic currents; (4) robust action potential frequency adaptation in response to depolarizing current injection in CA1 fast-spiking interneurons; and (5) no change in the number of pyramidal cells, but reduced number of parvalbumin positive interneurons.

SIGNIFICANCE:

Our data indicate that, in our genetic model of Atp1α3 mutation, there is increased excitability and marked dysfunction in GABAergic inhibition. This supports the performance of further investigations to determine if selective expression of the mutation in GABAergic and or glutamatergic neurons is necessary and sufficient to result in the behavioral phenotype.

KEYWORDS:

ATP1A3; D801N; alternating hemiplegia of childhood; epilepsy; parvalbumin

PMID:
29889309
DOI:
10.1111/epi.14441
[Indexed for MEDLINE]
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