Abstract
Sixty-one non-repetitive Enterobacter cloacae ESBL producers were collected at the Amiens University Hospital in France. Eight beta-lactam resistance phenotypes (a-h) and three aminoglycoside resistance phenotypes (i-k) were identified among these isolates, and 32 different pulsotypes were observed. Of these 61 isolates, 37 were sequenced and found to harbor beta-lactamases with a pI of 5.9 (TEM-4), 6.5 (TEM-24), 7.8 (SHV-4), 8.2 (SHV-12), 8.4 (CTX-M-1) and 8.0 (CTX-M-9). Four imipenem-resistant ESBL-producing E. cloacae isolates did not express the 38kDa OMP, indicating that this resistance is associated with porin deficiency.
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Anti-Bacterial Agents / pharmacology*
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Drug Resistance, Bacterial*
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Electrophoresis, Gel, Pulsed-Field
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Enterobacter cloacae / drug effects*
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Enterobacter cloacae / enzymology*
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Enterobacter cloacae / genetics
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Enterobacter cloacae / isolation & purification
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Enterobacteriaceae Infections / epidemiology
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Enterobacteriaceae Infections / microbiology*
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Female
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Humans
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Imipenem / pharmacology
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Infant
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Male
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Microbial Sensitivity Tests / methods
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Middle Aged
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Polymerase Chain Reaction
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Porins / genetics
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Porins / metabolism
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Sequence Analysis, DNA
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Young Adult
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beta-Lactamases / chemistry
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beta-Lactamases / classification*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Porins
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Imipenem
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beta-Lactamases