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Bioresour Technol. 2013 Sep;144:360-9. doi: 10.1016/j.biortech.2013.06.047. Epub 2013 Jun 28.

Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species.

Author information

1
Department of Food Science and Technology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USA. irsitepu@ucdavis.edu

Abstract

Oleaginous yeasts have been studied for oleochemical production for over 80 years. Only a few species have been studied intensely. To expand the diversity of oleaginous yeasts available for lipid research, we surveyed a broad diversity of yeasts with indicators of oleaginicity including known oleaginous clades, and buoyancy. Sixty-nine strains representing 17 genera and 50 species were screened for lipid production. Yeasts belonged to Ascomycota families, Basidiomycota orders, and the yeast-like algal genus Prototheca. Total intracellular lipids and fatty acid composition were determined under different incubation times and nitrogen availability. Thirteen new oleaginous yeast species were discovered, representing multiple ascomycete and basidiomycete clades. Nitrogen starvation generally increased intracellular lipid content. The fatty acid profiles varied with the growth conditions regardless of taxonomic affiliation. The dominant fatty acids were oleic acid, palmitic acid, linoleic acid, and stearic acid. Yeasts and culture conditions that produced fatty acids appropriate for biodiesel were identified.

KEYWORDS:

Biodiesel; Nitrogen starvation; Oleaginous yeast; Oleic acid; Triacylglycerides

PMID:
23891835
PMCID:
PMC3819430
DOI:
10.1016/j.biortech.2013.06.047
[Indexed for MEDLINE]
Free PMC Article

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