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Antiviral Res. 2011 Jan;89(1):43-53. doi: 10.1016/j.antiviral.2010.11.005. Epub 2010 Nov 18.

Long-term inhibition of HIV-1 replication with RNA interference against cellular co-factors.

Author information

1
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.

Abstract

In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability and inhibition of HIV-1 replication. For half of these candidate co-factors we obtained knockdown cell lines that are less susceptible to virus replication. For three co-factors (ALIX, ATG16 and TRBP) the cell lines were resistant to HIV-1 replication for up to 2 months. With these cells we could test the hypothesis that HIV-1 is not able to escape from RNAi-mediated suppression of cellular co-factors, which was indeed not detected.

PMID:
21093490
DOI:
10.1016/j.antiviral.2010.11.005
[Indexed for MEDLINE]

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