Format

Send to

Choose Destination

See 1 citation found by title matching your search:

J Biomed Opt. 2011 Apr;16(4):046018. doi: 10.1117/1.3569614.

Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.

Author information

1
Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, United Kingdom.

Abstract

We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation.

PMID:
21529087
PMCID:
PMC3321263
DOI:
10.1117/1.3569614
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center