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Biomed Pharmacother. 2016 May;80:207-212. doi: 10.1016/j.biopha.2016.03.003. Epub 2016 Mar 28.

Long noncoding RNA BDNF-AS protects local anesthetic induced neurotoxicity in dorsal root ganglion neurons.

Author information

1
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha 410002, China.
2
Department of Anesthesiology, Hunan Children's Hospital, Changsha 410007, China.
3
Department of Emergency, Hunan Provincial People's Hospital, Changsha 410002, China.
4
Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha 410002, China.
5
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: chengwei.song@aol.com.

Abstract

BACKGROUND:

Long noncoding RNA (lncRNA) BDNF-AS was recently identified to regulate neurotrophin signaling pathway. In this study, we examined the functional role of BDNF-AS in regulating local anesthetic-induced neurotoxicity in dorsal root ganglion (DRG) neurons.

METHODS:

Neonatal mouse DRG neurons were cultured in vitro, and treated with local anesthetic, bupivacaine, to induce neurotoxicity. The corresponding change in BDNF-AS expression level in DRG was probed by qRT-PCR. BDNF-AS was knocked down by siRNA in DRG. The effects of BDNF-AS downregulation on neurite regrowth, neuronal apoptosis and activating TrkB signaling pathway in bupivacaine-injured DRG neurons were probed by neurite outgrowth assay, TUNEL assay and western blot assay, respectively.

RESULTS:

During the process of bupivacaine-induce neurotoxicity in DRG, BDNF-AS was significantly upregulated in both dosage- and time- dependent manners. In DRG neurons, siRNA-mediated BDNF-AS downregulation promoted neurite outgrowth, reduced neuronal apoptosis, and phosphorylated TrkB signaling pathway after bupivacaine-induce neurotoxicity.

CONCLUSIONS:

BDNF-AS downregulation rescued local anesthetic-induce neurotoxicity in DRG neurons, probably through the activation of neurotrophin TrkB signaling pathway.

KEYWORDS:

BDNF-AS; Dorsal root ganglia neuron; Local anesthetic; Neurotoxicity; lncRNA

PMID:
27133058
DOI:
10.1016/j.biopha.2016.03.003
[Indexed for MEDLINE]

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