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J Lipid Res. 2017 Jan;58(1):42-59. doi: 10.1194/jlr.M068676. Epub 2016 Nov 23.

Localization of 1-deoxysphingolipids to mitochondria induces mitochondrial dysfunction.

Author information

1
Institute for Clinical Chemistry, University of Zurich, Zurich, Switzerland.
2
Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
3
Axonal Growth and Regeneration, German Center for Neurodegenerative Diseases, Bonn, Germany.
4
LIMES Life and Medical Sciences Institute, University of Bonn, Bonn, Germany.
5
Cyro-Electron Microscopy and Tomography, German Center for Neurodegenerative Diseases, Bonn, Germany.
6
Institute of Innate Immunity, University Hospital Bonn, Bonn, Germany.
7
Laboratory for Lipid Biochemistry and Protein Interactions, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium.
8
Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Lübeck, Germany.
9
LIMES Life and Medical Sciences Institute, University of Bonn, Bonn, Germany anke.penno@uni-bonn.de.

Abstract

1-Deoxysphingolipids (deoxySLs) are atypical sphingolipids that are elevated in the plasma of patients with type 2 diabetes and hereditary sensory and autonomic neuropathy type 1 (HSAN1). Clinically, diabetic neuropathy and HSAN1 are very similar, suggesting the involvement of deoxySLs in the pathology of both diseases. However, very little is known about the biology of these lipids and the underlying pathomechanism. We synthesized an alkyne analog of 1-deoxysphinganine (doxSA), the metabolic precursor of all deoxySLs, to trace the metabolism and localization of deoxySLs. Our results indicate that the metabolism of these lipids is restricted to only some lipid species and that they are not converted to canonical sphingolipids or fatty acids. Furthermore, exogenously added alkyne-doxSA [(2S,3R)-2-aminooctadec-17-yn-3-ol] localized to mitochondria, causing mitochondrial fragmentation and dysfunction. The induced mitochondrial toxicity was also shown for natural doxSA, but not for sphinganine, and was rescued by inhibition of ceramide synthase activity. Our findings therefore indicate that mitochondrial enrichment of an N-acylated doxSA metabolite may contribute to the neurotoxicity seen in diabetic neuropathy and HSAN1. Hence, we provide a potential explanation for the characteristic vulnerability of peripheral nerves to elevated levels of deoxySLs.

KEYWORDS:

ES-285; chemical synthesis; diabetes; inborn errors of metabolism; lipids/chemistry; metabolic syndrome; mitotoxicity; neurons; peripheral neuropathy; sphingolipids

PMID:
27881717
PMCID:
PMC5234710
DOI:
10.1194/jlr.M068676
[Indexed for MEDLINE]
Free PMC Article

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