Format

Send to

Choose Destination

See 1 citation found by title matching your search:

J Proteome Res. 2015 Oct 2;14(10):4282-95. doi: 10.1021/acs.jproteome.5b00447. Epub 2015 Sep 23.

Large Scale Discovery and De Novo-Assisted Sequencing of Cationic Antimicrobial Peptides (CAMPs) by Microparticle Capture and Electron-Transfer Dissociation (ETD) Mass Spectrometry.

Author information

1
Department of Biology, University of Florida , Gainesville, Florida 32611, United States.

Abstract

The identification and sequencing of novel cationic antimicrobial peptides (CAMPs) have proven challenging due to the limitations associated with traditional proteomics methods and difficulties sequencing peptides present in complex biomolecular mixtures. We present here a process for large-scale identification and de novo-assisted sequencing of newly discovered CAMPs using microparticle capture followed by tandem mass spectrometry equipped with electron-transfer dissociation (ETD). This process was initially evaluated and verified using known CAMPs with varying physicochemical properties. The effective parameters were then applied in the analysis of a complex mixture of peptides harvested from American alligator plasma using custom-made (Bioprospector) functionalized hydrogel particles. Here, we report the successful sequencing process for CAMPs that has led to the identification of 340 unique peptides and the discovery of five novel CAMPs from American alligator plasma.

KEYWORDS:

PEAKS db; alligator; antimicrobial; de novo sequencing; de novo-assisted sequencing; electron transfer dissociation; mass spectrometry; microparticles; peptides

PMID:
26327436
DOI:
10.1021/acs.jproteome.5b00447
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center