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Biosens Bioelectron. 2018 Nov 30;120:129-136. doi: 10.1016/j.bios.2018.08.025. Epub 2018 Aug 11.

Label-free ultrasensitive detection of breast cancer miRNA-21 biomarker employing electrochemical nano-genosensor based on sandwiched AgNPs in PANI and N-doped graphene.

Author information

1
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4. Electronic address: Razieh.salahandish@ucalgary.ca.
2
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran 1684613114, Iran. Electronic address: ghaffarinejad@iust.ac.ir.
3
Department of Biochemistry, Genetic and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran. Electronic address: skandar@pasteur.ac.ir.
4
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
5
Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran. Electronic address: kmajidzadeh@razi.tums.ac.ir.
6
Nanobioengineering Division, Department of Nanothechnology, School of New Technologies, Iran University of Science and Technology (IUST), P.O. Box 16846-13114, Tehran, Iran.
7
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Center for BioEngineering Research and Education, University of Calgary, Calgary, Alberta, Canada T2N 1N4. Electronic address: amir.sanatinezhad@ucalgary.ca.

Abstract

MicroRNAs (miRNAs) are small, endogenous, noncoding RNAs, shown to be expressed abnormally in many tumors and identified as predictive biomarkers for early diagnosis of several cancers including the breast. Therefore, the label-free and highly sensitive detection of miRNAs is of critical significance. In this work, a highly sensitive and label-free nano-genosensor is developed for the detection of miRNA-21, a known breast cancer biomarker, based on a specific architecture of nitrogen-doped functionalized graphene (NFG), silver nanoparticles (AgNPs), and polyaniline (PANI) that resulted in a remarkable effect on signal amplification. Following the successful functionalization of the nanocomposite and immobilization of the specific sequence of the aminated complementary oligonucleotide of miRNA-21, the detection was performed using differential pulse voltammetry (DPV). The oxidation peak current of the redox probe under optimal conditions was determined to monitor the event hybridization of miRNA-21 biomarker. Applying this highly sensitive and optimized nano-biosensor enabled detection of a wide dynamic range of 10 fM-10 µM with a sensitivity of 2.5 µA cm-2 and a low detection limit of 0.2 fM. This nano-biosensor also demonstrated highly reproducible results in the analysis of blood samples, with recoveries between 94% and 107%, and could be used for early detection of breast cancer by direct detection of the miRNA-21 in real clinical samples without any need to sample preparation, RNA extraction and/or amplification.

KEYWORDS:

Breast cancer; Electrochemical nano-genosensor; MiRNA-21; Nitrogen-doped modified graphene; Polyaniline; Silver nanoparticle

PMID:
30172235
DOI:
10.1016/j.bios.2018.08.025
[Indexed for MEDLINE]

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